The following approaches are novel, and require further testing in humans before being accepted as potential treatment options: Opioids can produce peripheral analgesic effects by activation of opioid receptors on sensory nerves. After intraperitoneal injections, 30% of the female mice died, compared with 3% of the male. Mesenchymal stem cells induce mature dendritic cells into a novel Jagged-2-dependent regulatory dendritic cell population. Of the time points we tested (6, 16, 24 h) our results showed that a 16-h post-intraperitoneal injection image scan provided the best contrast in mice regardless of age or disease burden. However, they found the migrated PCs was very few and these may attribute to: 1) the recipient are heathy rat. The bilateral femurs and tibias were aseptically excised, stripped of connective tissues and then stored in PBS. MSCs were isolated from C57BL/6 mice bone marrow as previously reported62. Proceedings of the National Academy of Sciences of the United States of America 99, 89328937, doi: 10.1073/pnas.132252399 (2002). World journal of gastroenterology 20, 1822818239, doi: 10.3748/wjg.v20.i48.18228 (2014). Because of the injection of cannabis, the patient was predicted to have some level of THC in the blood from absorption. CAS Indeed, Mariana et al. Heo, J. S., Choi, Y., Kim, H. S. & Kim, H. O. Intravenous vs intraperitoneal mesenchymal stem cells administration: what is the best route for treating experimental colitis? Highlighting the importance of choline metabolism . The data showed that the TSG-6 levels increased with time in both IP and IV injection groups, while in DSS control group, the TSG-6 level decreased from 48h to 72h. Although there is no significant difference among three injection groups at 48h after MSCs transplantation, the serum TSG-6 was significant higher in IP injection group compared with naive group (P=0.024). Google Scholar. Injection into egg 1 month 20 doses 1 dose 1 month 1 month Once 3.0 mL/kg 5 mL/kg 10 mL/kg 2.5 mL/kg 1 mL/kg O.^L/g Well tolerated Well tolerated Well tolerated Well tolerated Well tolerated Less mortality 1 \Jg Increase in mortality, decreased activity during righting reflex, running time, visual discrimination, and olfactory aversion test Horwitz, E. M. et al. However, for IV injection, we and others found most mice will lead increased mortality because of potential pulmonary cell embolus when the cell dose increase to 110722. If your cancer spreads to your abdomen or peritoneal space, IP chemotherapy can help target the cancer cells there. Intraperitoneal (IP) Injection (Injection of Sodium Pentobarbital . Fluorescent intensity was quantified and processed using the Living Image Software (Version 4.2; Caliper Life Science). The IP technique is an easy technique to master. Clinical use of bone marrow, bone marrow concentrate and expanded bone marrow mesenchymal stem cells in cartilage disease. Unable to load your collection due to an error, Unable to load your delegates due to an error. New chapters on interventional . Wang, N. et al. Rodents. Article Gastroenterology 114, 433440 (1998). Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). 5B). A disadvantage of using intraperitoneal injections is that they can have a large variability in effectiveness and misinjection. Stem Cells 29, 15721579, doi: 10.1002/stem.708 (2011). In DSS control FoxP3 positive cells only accounted for 1.51% of total cells, while the IP group showed 13.5% positive cells (P<0.001). While this route is an easy to master, quick, suitable for chronic treatments and with low impact of stress on laboratory rodents, there is a common concern that it may not be an acceptable route for drug administration in experimental studies. Histological evaluation was completed in a double-blind fashion by two independent pathologist. Vishnubalaji, R., Al-Nbaheen, M., Kadalmani, B., Aldahmash, A. Total RNA was extracted with the RNAeasy Plus kit (QIAGEN). Intraperitoneal Injection. We have proposed that safe and effective protection against the development of adult onset cancers may be achieved by vaccination against tissue-specific self-proteins that are "retired" from expression at immunogenic levels in normal tissues as we age, but are overexpressed in emerging tumors. Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in [citation needed]. In a previous study, 14 mice were exposed to SWCNTs through intravenous (IV) injection to determine whether there were any adverse effects caused by SWCNTs on the mouse brain and also to find a safe application dosage if possible. Vilquin, J. T. & Rosset, P. Mesenchymal stem cells in bone and cartilage repair: current status. Epidemiology and natural history of inflammatory bowel diseases. Note where each injection is made and subsequent injections should be made in slightly different spot than the previous injection. CAS It means IP injection is more feasible and reasonable for clinical settings. The Journal of infectious diseases 198, 456464, doi: 10.1086/590112 (2008). Human bronchial epithelial cells (BEAS-2B and 16HBE) were purchased from Shanghai Zhongqiao Xinzhou Biological Co., Ltd, and cultured in a complete medium supplemented with growth factors and .1% penicillin-streptomycin. Intramuscular. (A) Scheme of the treatment, the day of MSCs injection was defined as day 0. The latter is . It was determined that IP injections are a good option for Fenestra to quantify liver tumors in mice. . Surg Clin North Am 87, 727741, doi: 10.1016/j.suc.2007.03.004 (2007). Magnetically assisted intraperitoneal drug delivery for cancer chemotherapy. In IV group, most of MSCs were trapped in the lung, liver and spleen, while the cell that immigrated to the colon was not too much. Bethesda, MD 20894, Web Policies Tuning the Physicochemical Characteristics of Particle-Based Carriers for Intraperitoneal Local Chemotherapy. Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. Best way for mesenchymal stem cells transplantation for colitis. The formation of the aggregates probably limits access of the cells to the systemic circulation. Their findings showed that intraperitoneally injected MSCs would form aggregates with macrophages and lymphocytes in peritoneal cavity and remotely secreted TSG-6. Quantitative data are presented as the meanSD. After centrifuge, the cells were certificated and transferred to MSC expansion culture medium consisting of a-minimum essential medium (a-MEM; Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 100g/mL penicillin/streptomycin. In addition, IP showed more intense Ki-67 stain in bottom of the crypt, which suggested more active mucosa repair proliferation (Fig. Article Makhija, S. et al. Intra-abdominal adhesions can be a considerable source of morbidity and mortality. Kurtz, A. Mesenchymal stem cell delivery routes and fate. The fecal occult blood test on the day 3 also showed nearly complete absence of occult blood in IP group. A., Ong, J. F. & Karp, J. M. Mesenchymal stem cells: immune evasive, not immune privileged. One of the earliest recorded uses of IP injections involved the insemination of a guinea-pig in 1957. The results of this IP injection were the disappearance of the ascites associated with the rupture. Google Scholar. Evid Based Complement Alternat Med. Researchers has tried many creative ways to minimize these risks. And the loss of MSCs in the colon may attribute to many reasons, such as, wash out, cell death, or even rejection via the innate immune system. These cells were confirmed as bone marrow MSCs using flow-cytometric analysis and differential identification (Supplementary Figures S1 and S2). In patients with peritoneal surface malignancies, perioperative IP chemotherapy has been used with good results. & Correa, D. The MSC: an injury drugstore. Horizontal bars represent medians, boxes represent the 25th and 75th percentiles. National Library of Medicine Article CAS Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. 7 . However, the therapeutic impacts of cell delivery route that is critical for clinical translation are currently poorly understood. volume6, Articlenumber:30696 (2016) 107vs. 109 ; Fig. Condition. sharing sensitive information, make sure youre on a federal Article Essentials of Human Nutrition - Jun 13 2022 . Levy, O. et al. Intraperitoneal foreign bodies such as suture material, clips, and mesh also contribute to adhesion formation. ADS In contrast, GFP+ cells were showed in the lumen for AI group and lamina propria for IV group (Fig. Horwitz, E. M. et al. Peri/postnatal development in rats was impaired with reduced pup weights, increased stillbirths, and increased pup mortality concurrent with maternal toxicity after intraperitoneal administration of tazobactam alone at doses 320 mg/kg/day (2 times the human dose based on body surface area) or of the combination piperacillin and tazobactam at . Cancer Biother Radiopharm 28, 335342, doi: 10.1089/cbr.2012.1351 (2013). P values of<0.05 were considered statistically significant. Berberich, S., Forster, R. & Pabst, O. Very few reports pay attention to factors applied in the clinic, such as injection or exposure routes, which has highly impact on stem cell therapeutic efficiency for IBD12. humans have been associated with clinical pain, inflammatory lesions, and abscess formation in . In that same year, a study injected egg whites intraperitoneally into rats to study changes in "droplet" fractions in kidney cells. Mice are usu Stem cells international 2013, 732742, doi: 10.1155/2013/732742 (2013). Summers, R. W. Novel and future medical management of inflammatory bowel disease. Overall, many studies utilize IP injections to deliver therapeutics to lab animals due to the efficiency of the administration route. The levels of immunomodulatory cytokines were compared by real time-PCR and ELISA. Stem cells (Dayton, Ohio) 26, 279289, doi: 10.1634/stemcells.2007-0454 (2008). A study was conducted to determine the best route of administration to transplant mesenchymal stem cells for colitis. Kushnir, C. L., Fleury, A. C., Silver, D. F. & Spirtos, N. M. Intraperitoneal Catheter Placement: The Hammock Technique. In addition, even MSCs could formed aggregates in those harsh conditions, there still be a highly risk of self-removal. Generally, the mice were killed by cervical dislocation. Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat. Brown, S. L. et al. Zhang, B. et al. Background Saroglitazar is a novel PPAR-/ agonist with predominant PPAR- activity. The fluorescence imaging disclosed higher intensity of engrafted cells in inflamed colon and the corresponding mesentery lymph nodes (MLNs) in IP and AI groups than the IV group. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Road No. Some of the examples depict situations where IP injections are not ideal, while others prove the advantageous uses if this delivery method. Furthermore, the body weight changes displayed the less weight loss and quick weight gain of IP injection group and the maximum group difference among was reached on day 3 (Fig. In colonic MLNs, similar results were observed, the MSCs administration induced more FoxP3+ cells accumulation than the control, but the inter-group differences were not evident (Supplementary Figure S8). Disease recovery of DSS induced colitis after MSCs injection. Google Scholar. L.Z. For example, discharged accompany with stool or cleared out by lung microphage. Metabolite tracing, radioactive uptake experiments, and CRISPR screens demonstrate that FLVCR1 is the major plasma-membrane choline transporter. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. As mentioned above, a commonly used cell dose is 1106 cells/30g mouse, which would be equivalent to 33106/kg or approximately 2.3billion cells for a 70kg adult. MeSH Human peripheral blood lymphocytes (hu-PBL) can be adoptively transferred by intraperitoneal injection into mice with severe combined immunodeficiency (SCID). (C) Percentage of body weight changes over time. Background. 4 The scalp was disinfected and a midline incision was made in the skin. How to cite this article: Wang, M. et al. Sala, E. et al. Drug Deliv. Best way for mesenchymal stem cells transplantation for colitis. To determined cytokine in colon mucosa, protein extracts were isolated by homogenization of colonic segments (50mg tissue/mL) in 50mmol/L Tris-HCl, pH 7.4, with 0.5mmol/L dithiothreitol and 10 g/mL of a cocktail of proteinase inhibitors (Sigma). n=68 mice/group; n=3 for the control and naive (health mice) group. These findings could be advanced by studying how the route of injection affects the organs in the peritoneal cavity. Curr Opin Gastroenterol 30, 8496, doi: 10.1097/MOG.0000000000000031 (2014). Stem cell research & therapy 6, 55, doi: 10.1186/s13287-015-0066-5 (2015). For intraperitoneal administration, restrain the mouse with one hand and tilt the head backward to expose the abdomen. Currently, there are a handful of drugs that are delivered through intraperitoneal injection for chemotherapy. Thus, the aim of the current study was to find out the potential safety range of medical use. PubMed In 1996, intraperitoneal (IP) administration of cisplatin plus intravenous (IV) cyclophosphamide proved superior to both drugs given intravenously at the same doses-which, at the time, was the standard treatment in the United States. Wang Min, Liang Cong and Hu Hao contributed equally to this work. Best way for mesenchymal stem cells transplantation for colitis", "Intraperitoneal injections as an alternative method for micro-CT contrast enhanced detection of murine liver tumors", "Optimization of Intraperitoneal Injection Anesthesia in Mice: Drugs, Dosages, Adverse Effects, and Anesthesia Depth", "Intraperitoneal Injection Is Not Always a Suitable Alternative to Intravenous Injection for Radiotherapy", "Pharmacological principles of intraperitoneal and bidirectional chemotherapy", Transjugular intrahepatic portosystemic shunt, https://en.wikipedia.org/w/index.php?title=Intraperitoneal_injection&oldid=1142986299, Short description is different from Wikidata, Articles with unsourced statements from April 2015, Articles with unsourced statements from January 2022, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 5 March 2023, at 09:43. Use " or 1" needles to avoid penetrating abdominal organs. From two streams of research, experimental and clinical, stem cell therapy showed promising results. The expression level of TNF-, the most important pro-inflammatory cytokine, was markedly decreased in IP group, compared with AI (P=0.0174), IV (P=0.0019) and control group (P<0.001), respectively. Histopathological alterations in the mouse brain were detected after . Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Figure 1. The immunohistochemistry indicated higher repair proliferation (Ki-67) and more FoxP3+ cells accumulation of IP group. We even tried 1108 cell/mouse in IP and AI group and no immediate death was occurred. Shamsi M, Sedaghatkish A, Dejam M, Saghafian M, Mohammadi M, Sanati-Nezhad A. 2020 Apr;17(4):511-522. doi: 10.1080/17425247.2020.1736551. . This study compared intraperitoneal injections, intravenous injections, and anal injections. Our work used marrow-derived MSCs but not adipose-derived ones. 81371615), the Stake Key Laboratory Open Program of China (No. Liang, J. et al. Tests were read by an independent observer (Cong Liang) and scored 05 as shown by the color indicators provided by the manufacturer. The IV group showed 20.3% ki-67+ cells/crypt. IP, intraperitoneal injection; IV, intravenous injection; AI anal injection; n = 6-8 for treatment group, n = 3 for DSS control. At day 5, mice were randomized and injected with MSCs (1106, 200L in volume) by three delivery routes (IP, AI and IV). Unlike MSCs, they are restricted by migration because of receptors in the peritoneal cavity specially recognizing the PCs29,30. Every 1107 cells were incubated with 10mL DiR solution (PBS based containing 3.5g/mL dye and 0.5% ethanol) for 30min at 37C. PubMed Central Language links are at the top of the page across from the title. . A human study showed that after injection of HDL labeled with a CE tracer, most of the tracer that was ultimately found in the bile arrived there after transfer to apoB-containing lipoproteins, . Cite this article. In this study, the therapeutic efficiency of three different MSCs delivery routes (IP, AI and IV) were compared on DSS-induced colitis mouse model. This inconsistency may attributed to different MSCs types.

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